For us, the valuables we keep locked up are:
1. The laptop
2. Our wallets. We leave most of our credit cards and stuff at home, but we always travel with at least 2 different credit cards and about $200 in cash. We only carry ID, the Disney Visa card, and a little cash to the parks. The remaining cash & credit cards stay in our wallets in the safe.
3. Any jewelry I'm not wearing. I don't usually bring extra jewelry but sometimes my fingers swell from the heat so I'll take my rings off and leave them in the safe on really hot days.
4. All of our mousekeeping tip envelopes as they're already loaded with cash.
5. If there's room, I'll put my purse in there, too. I usually carry a D&B Disney purse for my every-day purse but I only take a tiny cell phone cross-body pouch to the parks. If my purse doesn't fit in the safe, I just keep it in a piece of luggage. I don't really have anything of value in the purse, so I'm not overly concerned about it, but I don't want to make it easy to just pick up and walk out of the room with it.
Most of these things just "live" in the safe, but we do need to get things out of the safe every day so we need to remember to close it up before we leave the room in the morning. I forgot to close it one morning a few years ago -- and of course it was a day that I had left my rings in it. I knew there was over $15,000 worth of "valuables" in the safe that day and went into a complete panic at lunch when I realized that I had left the safe standing wide open. At that point, I knew it was probably too late to go back and close it -- mousekeeping had probably already been in our room. I was very happy to get back to the room that night and see that nothing had been touched in the safe. Now I check the safe 10 times before we leave the room.